Prevalence and Phylogenetic Analysis of Babesia ovis Isolated from Sheep and Goats in Erbil Province, Kurdistan Region- Iraq
DOI:
https://doi.org/10.25156/ptj.v10n2y2020.pp98-104Keywords:
18S SSU rRNA, Babesiosis, Erbil province, Phylogeny, Tick-borneAbstract
A total of 143 blood smears were obtained from sheep and goats (n = 98) and (n = 45), respectively, by jugular venipuncture between February 2019 and December 2019. Samples were examined for parasite occurrences. Results reveal that the prevalence of infection by the parasite was 12.24% and 11.11%, respectively (microscopically), while 20.41% sheep and 17.78% goats (molecular-based identification using 18S SSU rRNA sequence). Both methods revealed the specificity and sensitivity of the diagnostic assays. Phylogenetic analysis of Babesia ovis (MN309736-MN309745) displays that the pathogens identified in this study are clustered together, which indicating similar molecular characteristics. Overall, these results provide a better understanding that Babesiosis is highly prevalent in the study area and continuous monitoring of tick-borne pathogen in sheep and goats are essentially required in Erbil Province.
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