Epidemiological Study and Molecular-Based Identification of Enterobius vermicularis Among Qushtapa Refugee Camp Children
DOI:
https://doi.org/10.25156/ptj.v12n2y2022.pp%25pKeywords:
key words: Entrobius vermicularis, entrobiasis, refuge camp, Qushtapa, 28S rDNAAbstract
Abstract
Two hundred and six (206) stool samples were taken from children aged 1 to 12 resident in Qushtapa refugee camp. Samples were collected from beginning of October 2019 until the end of February 2020. A questionnaire was used to collect data on socio-demographic status. Result reveals an overall prevalence of infection was (33 %). Children aged between 6 to 12 have higher prevalence of infection (43.3%) in comparison with those aged less than 6 years (22.5%). Several factors have statistically significant differences in proportion related to enterobiasis infection. Factors are age (p-value=0.002), hand washing (p-value=0.039), mother education level (p-value=0.323), family size (p-value=0.424), nailed finger (p-value=0.57). Molecular analysis was carried out using 28S rDNA, isolated from adult female and primers targeting the area of 28S rDNA. Analysis of the original sequence using the universal primers of the sample of the study divulged that the parasite belonged to the species Enterobius vermicularis (sequence similarity was performed using BLAST from NCBI and pairwise alignment=%100).
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