Enzymatic Oxidative Stress Biomarkers Alterations in Treated Addicted Female Rats

Authors

  • Twana Mustafa Medical laboratory Technology Dept./ Health technical College/Hawler Polytechnic University

DOI:

https://doi.org/10.25156/ptj.2018.8.2.208

Keywords:

Cannabis, Oxidative stress, Female rats

Abstract

Background and Objective: Addiction with Cannabis represents one of the major spreading phenomena worldwide. The objective of this study is to evaluate the oxidative stress enzyme markers and lipid peroxidation marker in cannabis administered female rats via intraperitoneal injection. MATERIALS AND METHODS: Thirty female rats were randomly allocated into three groups. First group regarded as control, while the animals of the second and third groups were daily injected intraperitoneally with (5 and 10 mg Cannabis/kg b.w.) for seven successive days. Enzymatic activities of alkaline phosphatase (ALP), Xanthine Oxidase (XO), Glucose-6- phosphate dehydrogenase (G6PDH) and lactate dehydrogenase (LDH) in serum were analyzed, and concentration of Malondialdehyde (MDA) was assayed. RESULTS: The activity of ALP, G6PDH and LDH significantly affected, whereas XO not affected. The lipid peroxidation marker; MDA significantly elevated in high dose treatment. CONCLUSION: This finding suggests that intraperitoneal injection of cannabis causes alterations in oxidation process

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References

ABUSE, S. 2014. Mental Health Services Administration,(SAMHSA). Results from the 2012 national survey on drug use and health: Summary of national findings. Rockville, MD: Substance Abuse and Mental Health Services Administration; 2013. NSDUH Series H-46, HHS Publication No.(SMA) 13–4795. Substance Abuse and Mental Health Services Administration.

ADAMS, I. B. & MARTIN, B. R. 1996. Cannabis: pharmacology and toxicology in animals and humans.

Addiction, 91, 1585-614.

AGARWAL, A., APONTE-MELLADO, A., PREMKUMAR, B. J., SHAMAN, A. & GUPTA, S. 2012. The effects of

oxidative stress on female reproduction: a review. Reproductive Biology and Endocrinology, 10, 49.

ATHANASIOU, A., CLARKE, A. B., TURNER, A. E., KUMARAN, N. M., VAKILPOUR, S., SMITH, P. A., BAGIOKOU, D., BRADSHAW, T. D., WESTWELL, A. D., FANG, L., LOBO, D. N., CONSTANTINESCU,

C. S., CALABRESE, V., LOESCH, A., ALEXANDER, S. P., CLOTHIER, R. H., KENDALL, D. A. & BATES, T.

E. 2007. Cannabinoid receptor agonists are mitochondrial inhibitors: a unified hypothesis of how cannabinoids modulate mitochondrial function and induce cell death. Biochem Biophys Res Commun, 364, 131-7.

Avraham Y, Zolotarev O, Nikolaos C. Grigoriadis, M.D., Theofilos Pautahidis, Magen I, Lia Vorobiav. (2008). Cannabinoids and Capsaicin Improve Liver Function Following Thioacetamide-Induced Acute Injury in Mice. American Journal of Gastroenterology. .1572-0241

KUMAR, R. N., CHAMBERS, W. A. & PERTWEE, R. G. 2001. Pharmacological actions and therapeutic uses of cannabis and cannabinoids. Anaesthesia, 56, 1059-68.

MALLAT, A. & LOTERSZTAJN, S. 2010. Endocannabinoids and their role in fatty liver disease. Dig Dis, 28, 261-6.

MANDAL, T. K. & DAS, N. S. 2010. Testicular toxicity in cannabis extract treated mice: association with oxidative stress and role of antioxidant enzyme systems. Toxicol Ind Health, 26, 11-23.

MECHOULAM, R. & HANUS, L. 2000. A historical overview of chemical research on cannabinoids. Chem Phys Lipids, 108, 1-13.

MUKHTA, A. H., ELBAGIR N. M. & GUBARA A. A. (2011). THE EFFECT OF CANNABIS SATIVA ON CERTAIN

ENZYMES OF CLINICAL SIGNIFICANCE IN RATS AND MEN. Journal of Pharmacognosy. pp-10-13 MUSLIH, R. D. A.-N., O.M. AND AL-ZAMELY, M.A. 2002. The level of Malondialdehyde after activation

with (H2O2 and CuSO4) and inhibited by Desferoxamine and Molsidomine in the serum of patients with acute myocardial infection. Nat. J. Chem., 5, 148-149.

NAGARKATTI, P., PANDEY, R., RIEDER, S. A., HEGDE, V. L. & NAGARKATTI, M. 2009. Cannabinoids as

novel anti-inflammatory drugs. Future Med Chem, 1, 1333-49.

Owolabi J.O, Olatunji, S.Y. and A.J. Olanrewaju. 2017. Caffeine and Cannabis Effects on Vital Neurotransmitters and Enzymes in the Brain Tissue of Juvenile Experimental Rats. Ann Neurosci. 24(2): 65–73.

PARFIENIUK, A. & FLISIAK, R. Role of cannabinoids in chronic liver diseases, World J Gastroenterol. 2008 Oct 28;14(40):6109-14.

Pertwee RG. The diverse CB1 and CB2 receptor pharmacology of three plant cannabinoids: delta9- tetrahydrocannabinol, cannabidiol and delta9-tetrahydrocannabivarin. Br J Pharmacol 2008;153:199–215

QURAISHI, R., JAIN, R., CHATTERJEE, B. & VERMA, A. 2013. Laboratory profiles of treatment-seeking subjects with concurrent dependence on cannabis and other substances: a comparative study. Int J High Risk Behav Addict, 2, 107-11.

SARAFIAN, T. A., MAGALLANES, J. A. M., SHAU, H., TASHKIN, D. & ROTH, M. D. 1999. Oxidative stress

produced by marijuana smoke: an adverse effect enhanced by cannabinoids. American journal of respiratory cell and molecular biology, 20, 1286-1293.

VOLKOW, N. D., BALER, R. D., COMPTON, W. M. & WEISS, S. R. 2014. Adverse health effects of marijuana use. N Engl J Med, 370, 2219-27.

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Published

2023-07-20

How to Cite

Mustafa, T. (2023). Enzymatic Oxidative Stress Biomarkers Alterations in Treated Addicted Female Rats. Polytechnic Journal, 8(2), 487-495. https://doi.org/10.25156/ptj.2018.8.2.208

Issue

Vol. 8 No. 2 (2018)

Section

Review Articles

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Copyright (c) 2023 Twana Mustafa

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