EVALUATION THE EFFICACY OF DILUTED CHLOROXYLENOL AGAINST BIOFILM PRODUCING PSEUDOMONAS AERUGINOSA IN CLINICAL ISOLATES
DOI:
https://doi.org/10.25156/ptj.2018.8.2.239Keywords:
Biofilm formation; Chloroxylenol; Disinfectant; Drug resistance; P.aeruginosa.Abstract
The extensive use of disinfectant and their causing in dissemination at the hospitals can contribute to alterations in bacteria leading to the expansion of highly resistant microorganisms to antibacterial agents. The mechanisms of resistance in bacteria are similar for both antibacterial agents and disinfectant. The main objective of this study was to assess the activity of the various dilution of the chloroxylenol as an ordinarily used disinfectant against P. aeruginosa at hospitals and their association to biofilm production. This study was carried out on 91 P. aeruginosa obtained from different clinical specimens at hospitals in Erbil city. All clinical isolates of P. aeruginosa were screened for biofilm formation in different concentration of disinfectants. The activity of chloroxylenol on P. aeruginosa to the biofilm was found to be concentration reliant. The isolates showed to be a non-biofilm producer to dilution factor of 1:10 and 1:20, while in the range between 1:40 to1:160 the ability was higher to biofilm formation. The maximum inhibition rate of chloroxylenol was documented 43% of isolates for 1/2 MIC, while the lowest inhibition 17% was established for 1/32 MIC. It might be probable that P. aeruginosa modifies to resistant which leads to their survival even at high concentrations of disinfectant. Therefore, it is observable that resistance to disinfectant especially in the hospital settings could be due to multi-resistance bacteria then it can be easily conveyed to patients who admitted to hospital.
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