Effect of Streptomyces xantholiticus on Rice Blast Disease Reduction and Enzyme Activity
DOI:
https://doi.org/10.25156/ptj.v11n1y2021.pp112-117Keywords:
Pyricularia oryzae, Peroxidase enzyme, Polyphenol oxidase, Plant growth, Biological controlAbstract
The rice blast infection triggered by Pyricularia oryzae Cav. is a grave issue restraining rice yields globally. Biological regulation has been seen as an encouraging option to such chemical fungicides. The biological control proficiency of 24 isolates of Streptomyces against P. oryzae (KT693184.1) has been examined. Analysis of defense enzymes such as polyphenol, peroxidase (PO), and total protein and phenol content was performed. The Streptomyces isolate that was determined to have maximum effectiveness was determined to be Streptomyces xantholiticus. The sequence was sent to the NCBI vide accession number MW029942. The S. xantholiticus isolate inhibited P. oryzae 92% using dual culture tests. The greenhouse experiments showed that the disease gravity in rice plants with S. xantholiticus isolate was considerably lower compared to plants sans the treatment. The S. xantholiticus isolate was analyzed for its effects concerning the activity of polyphenol and Po enzymes as well as the total protein content present in rice leaves under greenhouse conditions in both presence and absence of P. oryzae. It was found that rice species treated with the S. xantholiticus isolate had enhanced resistance to the rice blast pathogen along with increasing activity levels of the polyphenol and PO enzymes. There was a direct impact on pathogen growth along with the defense response triggered in the plants as a result of the treatment.
Downloads
References
Abo-Elyousr, K. A. and H. H. El-Hendawy. 2008. Integration of Pseudomonas fluorescens and acibenzolar-S-methyl to control bacterial spot disease of tomato. Crop Protect. 27(7): 1118-1124.
Adhilakshmi, M. and R. Velazhahan. 2013. Analysis of peroxidase and polyphenol oxidase isozyme profiles in groundnut plants treated with Streptomyces sp. Madras Agric. J. 100: 879-882.
Adhilakshmi, M., V. Paranidharan, D. Balachandar, K. Ganesamurthy and R. Velazhahan. 2014. Suppression of root rot of mung bean (Vigna radiata L.) by Streptomyces sp. is associated with induction of peroxidase and polyphenol oxidase. Arch. Phytopathol. Plant Protect. 47(5): 571-583.
Adnan, M., W. Islam, A. Shabbir, K. A. Khan, H. A. Ghramh, Z. Huang, H. Y. Chen and G. D. Lu. 2019. Plant defense against fungal pathogens by antagonistic fungi with Trichoderma in focus. Microb. Pathogen. 129: 7-18.
Arora, R. and M. E. Wisniewski. 1994. Cold acclimation in genetically related (sibling) deciduous and evergreen peach (Prunus persica (L.) Batsch) II. A 60-kilodalton bark protein in cold-acclimated tissues of peach is heat stable and related to the dehydrin family of proteins. Plant Physiol. 105(1): 95-101.
Awla, H. K. and T. S. Rashid. 2020. HPLC fractionation: A comparative analysis of anti-fungal compounds from different Streptomyces isolates inhibiting Colletotrichum acutatum. Biocatal. Agric. Biotechnol. 27: 101688.
Awla, H. K., J. Kadir, R. Othman, T. S. Rashid and M. Y. Wong. 2016. Bioactive compounds produced by Streptomyces sp. Isolate UPMRS4 and antifungal activity against Pyricularia oryzae. Am. J. Plant Sci. 7(7): 1077-1085.
Awla, H. K., J. Kadir, R. Othman, T. S. Rashid, S. Hamid and M. Y. Wong. 2017. Plant growth-promoting abilities and biocontrol efficacy of Streptomyces sp. UPMRS4 against Pyricularia oryzae. Biol. Control. 112: 55-63.
Bibb, M. J. 2005. Regulation of secondary metabolism in streptomycetes. Curr. Opin. Microbiol. 8(2): 208-215.
Bressan, W. and J. E. F. Figueiredo. 2008. Efficacy and doseresponse relationship in biocontrol of Fusarium disease in maize by Streptomyces spp. Eur. J. Plant Pathol. 120(3): 311-316.
Chandra, A. and R. K. Bhatt. 1998. Biochemical and physiological response to salicylic acid in relation to the systemic acquired resistance. Photosynthetica. 35(2): 255-258.
Chen, H. L., B. T. Chen, D. P. Zhang, Y. F. Xie and Q. Zhang. 2001. Pathotypes of Pyricularia grisea in rice fields of central and Southern China. Plant Dis. 85(8): 843-850.
Christopher, D. J., T. S. Raj, S. U. Rani and R. Udhayakumar. 2010. Role of defense enzymes activity in tomato as induced by Trichoderma virens against Fusarium wilt caused by Fusarium oxysporum f sp. Lycopersici. J. Biopest. 3(1): 158.
Chung, W. C., J. W. Huang and H. C. Huang. 2005. Formulation of a soil biofungicide for control of damping-off of Chinese cabbage (Brassica chinensis) caused by Rhizoctonia solani. Biol. Control. 32(2): 287-294.
Couch, B. C., I. Fudal, M. H. Lebrun, D. Tharreau, B. Valent, P. Van Kim, J. L. Nottéghem and L. M. Kohn. 2005. Origins of hostspecific populations of the blast pathogen Magnaporthe oryzae in crop domestication with subsequent expansion of pandemic clones on rice and weeds of rice. Genetics. 170(2): 613-630.
Dean, R. A., N. J. Talbot, D. J. Ebbole, M. L. Farman, T. K. Mitchell, M. J. Orbach, M. Thon, R. Kulkarni, J. R. Xu, H. Pan and N. D. Read. 2005. The genome sequence of the rice blast fungus Magnaporthe grisea. Nature. 434(7036): 980-986.
González-Franco, A. C. and L. Robles-Hernandez. 2009. Actinomycetes as biological control agents of phytopathogenic fungi. Tecnociencia Chihuahua. 3(2): 64-73.
Karthikeyan, V. and S. S. Gnanamanickam. 2008. Biological control of Setaria blast (Magnaporthe grisea) with Bacteria strains. Crop Protect. 27: 263-267.
Kashif, M. D., V. Kumar, V. N. Kalpana and V. D. Rajeswari. 2016. Phylogenetic diversity and biological activity of actinomycetes isolated from Gulf of Mannar, Tamil Nadu, India. Pharm. Lett. 8: 16-24.
Law, J. W. F., H. L. Ser, T. M. Khan, L. H. Chuah, P. Pusparajah, K. G. Chan, B. H. Goh and L. H. Lee. 2017. The potential of Streptomyces as biocontrol agents against the rice blast fungus, Magnaporthe oryzae (Pyricularia oryzae). Front. Microbiol. 8: 3.
Li, Q., Y. Jiang, P. Ning, L. Zheng, J. Huang, G. Li, D. Jiang and T. Hsiang. 2011. Suppression of Magnaporthe oryzae by culture filtrates of Streptomyces globisporus JK-1. Biol. Control. 58(2): 139-148.
Mahadevan, A. and R. Sridhar. 1982. Methods in Physiological Plant Pathology. Sivakami Publication, India. p316.
Mahato, S., S. Bhuju and J. Shrestha. 2018. Effect of Trichoderma viride as biofertilizer on growth and yield of wheat Malays. J. Sustain. Agric. 2(2): 1-5.
Morkunas, I. and J. Gmerek. 2007. The possible involvement of peroxidase in defense of yellow lupine embryo axes against Fusarium oxysporum. J. Plant Physiol. 164(2): 185-194.
Noreen, H., N. Semmar, M. Farman and J. S. McCullagh. 2017. Measurement of total phenolic content and antioxidant activity of aerial parts of medicinal plant Coronopus didymus. Asian Pac. J. Trop. Med. 10(8): 792-801.
Ojha, S. and N. C. Chatterjee. 2012. Induction of resistance in tomato plants against Fusarium oxysporum f. sp. Lycopersici mediated through salicylic acid and Trichoderma harzianum. J. Plant Protect. Res. 52(2): 220-225.
Prabavathy, V. R., N. Mathivanan and K. Murugesan. 2006. Control of blast and sheath blight disease of rice using antifungal metabolites produced by Streptomyces sp. PM5. Biol. Control. 39: 313-319.
Pradeep, T. and N. D. Jambhale. 2002. Relationship between phenolics, polyphenol oxidase and peroxidases and resistance to powdery mildew in Zizhyphus. Indian Phytopathol. 55(2): 195-196.
Rahman, M., M. Z. Islam, M. Islam and A. Ul Islam. 2011. Antibacterial activities of actinomycete isolates collected from soils of Rajshahi, Bangladesh. Biotechnol. Res. Int. 2011: 857925.
Ramesh, R., A. A. Joshi and M. P. Ghanekar. 2009. Pseudomonads: Major antagonistic endophytic bacteria to suppress bacterial wilt pathogen, Ralstonia solanacearum in the eggplant (Solanum melongena L.). World J. Microbiol. Biotechnol 25(1): 47-55.
Rhee, K. H. 2003. Purification and identification of an antifungal agent from Streptomyces sp. KH-614 antagonistic to rice blast fungus, Pyricularia oryzae. J. Microbiol. Biotechnol. 13(6): 984-988.
Sadeghi, A., P. Koobaz, H. Azimi, E. Karimi and A. R. Akbari. 2017. Plant growth promotion and suppression of Phytophthora drechsleri damping-off in cucumber by cellulase-producing Streptomyces. BioControl. 62(6): 805-819.
Sha, Y., Q. Wang and Y. Li. 2016. Suppression of Magnaporthe oryzae and interaction between Bacillus subtilis and rice plants in the control of rice blast. SpringerPlus. 5(1): 1238.
Shahidi, B. G. H., P. Rashid Farrokhi, S. Aghighi, L. Shahidi Bonjar and A. Aghelizadeh. 2005. Antifungal characterization of actinomycetes isolated from Kerman, Iran and their future prospects in biological control strategies in greenhouse and field conditions. Plant Pathol. J. 4(1): 78-84.
Sreedevi, B., M. Charitha Devi and D. V. R. Saigopal. 2011. Isolation and screening of effective Trichoderma spp. against the root rot pathoge Macrophomina phaseolina. J. Agric. Technol. 7(3): 623-635.
Sunpapao, A., T. Chairin and S. I. Ito. 2018. The biocontrol by Streptomyces and Trichoderma of leaf spot disease caused by Curvularia oryzae in oil palm seedlings. Biol. Control. 123: 36-42.
Vidhyasekaran, P. 2004. Concise Encyclopedia of Plant Pathology. CRC Press, United States.
Zarandi, M. E., G. S. Bonjar, F. P. Dehkaei, S. A. Moosavi, P. R. Farokhi and S. Aghighi. 2009. Biological control of rice blast (Magnaporthe oryzae) by use of Streptomyces sindeneusis isolate 263 in greenhouse. Am. J. Appl. Sci. 6(1): 194-199.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Hayman K. Awla
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Authors who publish with this journal agree to the following terms:
1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License [CC BY-NC-ND 4.0] that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.
2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.
3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
Polytechnic Journal ; A Periodical Open Access Scientific Journal